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VEGF and MMP ‐9: biomarkers for canine lymphoma
Author(s) -
Aresu L.,
Aricò A.,
Comazzi S.,
Gelain M. E.,
Riondato F.,
Mortarino M.,
Morello E.,
Stefanello D.,
Castagnaro M.
Publication year - 2014
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/j.1476-5829.2012.00328.x
Subject(s) - vascular endothelial growth factor , lymphoma , canine lymphoma , chemotherapy , matrix metalloproteinase , medicine , vegf receptors , zymography , angiogenesis , non hodgkin's lymphoma , stage (stratigraphy) , immunophenotyping , pathology , gastroenterology , cancer research , endocrinology , immunology , biology , antigen , paleontology
Vascular endothelial growth factor ( VEGF ) and metalloproteinase ( MMP ) 2 and 9 are useful biomarkers in human lymphoma. During cancerogenesis, transforming growth factor beta ( TGF ‐β) stimulates VEGF and MMPs production. VEGF and TGF ‐β plasma levels were tested by ELISA , MMP ‐2 and MMP ‐9 by gelatine zymography in 37 dogs with lymphoma, 13 of which were also monitored during chemotherapy. Ten healthy dogs served as control. Lymphoma dogs showed higher act‐ MMP ‐9 ( P < 0.01) and VEGF ( P < 0.05), and lower TGF ‐β than controls, and a positive correlation between act‐ MMP ‐9 and VEGF ( P < 0.001). Act‐ MMP ‐9 and VEGF were significantly higher in T‐cell lymphomas, and in stage V compared with stages III–IV disease, regardless of immunophenotype. VEGF was higher in high‐grade compared with low‐grade T‐cell lymphomas. No correlation was found between cytokines levels at presentation and outcome. During chemotherapy, act‐ MMP ‐9 and VEGF decreased in B‐cell lymphomas ( P < 0.01), suggesting a possible predictive role in this group of dogs.