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Detection of the tumour suppressor gene TP53 and expression of p53, Bcl‐2 and p63 proteins in canine transmissible venereal tumour
Author(s) -
Stockmann D.,
Ferrari H. F.,
Andrade A. L.,
Cardoso T. C.,
Luvizotto M. C. R.
Publication year - 2011
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/j.1476-5829.2010.00260.x
Subject(s) - messenger rna , gene , in situ hybridization , biology , reverse transcriptase , homologous chromosome , polymerase chain reaction , gene expression , apoptosis , rna , neoplasm , microbiology and biotechnology , cell , pathology , real time polymerase chain reaction , p53 protein , medicine , genetics
Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl‐2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT‐PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl‐2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.