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Proteomic identification and profiling of canine lymphoma patients *
Author(s) -
Ratcliffe L.,
Mian S.,
Slater K.,
King H.,
Napolitano M.,
Aucoin D.,
Mobasheri A.
Publication year - 2009
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/j.1476-5829.2008.00165.x
Subject(s) - lymphoma , surface enhanced laser desorption/ionization , predictive value , medicine , pathology , mass spectrometry , chromatography , chemistry , tandem mass spectrometry , protein mass spectrometry
This study employed proteomic and bioinformatic approaches to identify serum biomarkers in canine lymphoma patients. Chilled serum samples derived from non‐lymphoma ( n = 92) and lymphoma ( n = 87) patients were shipped from first opinion veterinary practices, subjected to ion exchange chromatography and analysed by surface‐enhanced laser desorption ionization mass spectrometry. Nineteen serum protein peaks were identified between the two groups as being significantly different ( P < 0.05) based upon their normalized ion intensities. Two biomarkers were identified that were capable of differentiating lymphoma and non‐lymphoma patients. Analysis of the test data provided a positive predictive value (PPV) of 82%. A clinical follow‐up study was carried out on 96 canine patients suspected of having lymphoma. Evaluation of this data gave a specificity value of 91%, sensitivity of 75%, PPV of 80% and negative predictive value of 88%. In conclusion, the expression pattern of two serum biomarkers has enabled serum samples to be classified into either lymphoma or non‐lymphoma categories.