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Effects of palmitoylation of Cys 415 in helix 8 of the CB 1 cannabinoid receptor on membrane localization and signalling
Author(s) -
Oddi Sergio,
Dainese Enrico,
Sandiford Simone,
Fezza Filomena,
Lanuti Mirko,
Chiurchiù Valerio,
Totaro Antonio,
Catanzaro Giuseppina,
Barcaroli Daniela,
De Laurenzi Vincenzo,
Centonze Diego,
Mukhopadhyay Somnath,
Selent Jana,
Howlett Allyn C,
Maccarrone Mauro
Publication year - 2012
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.2011.01658.x
Subject(s) - palmitoylation , g protein coupled receptor , biology , microbiology and biotechnology , receptor , g protein , adenylyl cyclase , lipid raft , fluorescence recovery after photobleaching , hek 293 cells , cannabinoid , signal transduction , biochemistry , cysteine , membrane , enzyme
The CB(1) cannabinoid receptor is regulated by its association with membrane microdomains such as lipid rafts. Here, we investigated the role of palmitoylation of the CB(1) receptor by analysing the functional consequences of site-specific mutation of Cys(415) , the likely site of palmitoylation at the end of helix 8, in terms of membrane association, raft targeting and signalling.