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Compartmentalization of endocannabinoids into lipid rafts in a microglial cell line devoid of caveolin‐1
Author(s) -
Rimmerman Neta,
Bradshaw Heather B,
Kozela Ewa,
Levy Rivka,
Juknat Ana,
Vogel Zvi
Publication year - 2012
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.2011.01380.x
Subject(s) - lipid raft , ethanolamine , sphingomyelin , lipid microdomain , biochemistry , anandamide , caveolae , chemistry , biology , cannabinoid receptor , receptor , cell , cholesterol , membrane , agonist
N-acyl ethanolamines (NAEs) and 2-arachidonoyl glycerol (2-AG) are endogenous cannabinoids and along with related lipids are synthesized on demand from membrane phospholipids. Here, we have studied the compartmentalization of NAEs and 2-AG into lipid raft fractions isolated from the caveolin-1-lacking microglial cell line BV-2, following vehicle or cannabidiol (CBD) treatment. Results were compared with those from the caveolin-1-positive F-11 cell line.