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Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A 1 receptor ligands
Author(s) -
Baker Jillian G,
Middleton Richard,
Adams Luke,
May Lauren T,
Briddon Stephen J,
Kellam Barrie,
Hill Stephen J
Publication year - 2010
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.2009.00488.x
Subject(s) - linker , fluorophore , adenosine , chemistry , agonist , chinese hamster ovary cell , biophysics , radioligand , fluorescence , receptor , adenosine receptor , biochemistry , biology , physics , quantum mechanics , computer science , operating system
The introduction of fluorescence-based techniques, and in particular the development of fluorescent ligands, has allowed the study of G protein-coupled receptor pharmacology at the single cell and single molecule level. This study evaluated how the physicochemical nature of the linker and the fluorophore affected the pharmacological properties of fluorescent agonists and antagonists.