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Clovamide and rosmarinic acid induce neuroprotective effects in in vitro models of neuronal death
Author(s) -
Fallarini S,
Miglio G,
Paoletti T,
Minassi A,
Amoruso A,
Bardelli C,
Brunelleschi S,
Lombardi G
Publication year - 2009
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.2009.00213.x
Subject(s) - excitotoxicity , rosmarinic acid , neuroprotection , programmed cell death , oxidative stress , intracellular , glutamate receptor , glutathione , chemistry , pharmacology , receptor , biology , biochemistry , apoptosis , antioxidant , enzyme
Background and purpose: Phenolic compounds exert cytoprotective effects; our purpose was to investigate whether the isosteric polyphenolic compounds clovamide and rosmarinic acid are neuroprotective. Experimental approach: Three in vitro models of neuronal death were selected: (i) differentiated SH‐SY5Y human neuroblastoma cells exposed to tert ‐butylhydroperoxide ( t ‐BOOH), for oxidative stress; (ii) differentiated SK‐N‐BE(2) human neuroblastoma cells treated with L‐glutamate, for excitotoxicity; and (iii) differentiated SH‐SY5Y human neuroblastoma cells exposed to oxygen‐glucose deprivation/reoxygenation, for ischaemia–reperfusion. Cell death was evaluated by lactate dehydrogenase measurements in the cell media, while the mechanisms underlying the effects by measuring: (i) t ‐BOOH‐induced glutathione depletion and increase in lipoperoxidation; and (ii) L‐glutamate‐induced intracellular Ca 2+ overload (fura‐2 method) and inducible gene expression ( c‐fos , c‐jun ), by reverse transcriptase‐PCR. The ability of compounds to modulate nuclear factor‐κB and peroxisome proliferator‐activated receptor‐γ activation was evaluated by Western blot in SH‐SY5Y cells not exposed to harmful stimuli. Key results: Both clovamide and rosmarinic acid (10–100 µmol·L −1 ) significantly protected neurons against insults with similar potencies and efficacies. The EC 50 values were in the low micromolar range (0.9–3.7 µmol·L −1 ), while the maximal effects ranged from 40% to −60% protection from cell death over untreated control at 100 µmol·L −1 . These effects are mediated by the prevention of oxidative stress, intracellular Ca 2+ overload and c‐fos expression. In addition, rosmarinic acids inhibited nuclear factor‐κB translocation and increased peroxisome proliferator‐activated receptor‐γ expression in SH‐SY5Y cells not exposed to harmful stimuli. Conclusion and implications: Clovamide and rosmarinic acid are neuroprotective compounds of potential use at the nutritional/pharmaceutical interface.