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Functional, endogenously expressed 5‐hydroxytryptamine 5‐ht 7 receptors in human vascular smooth muscle cells
Author(s) -
Schoeffter P.,
Ullmer C.,
Bobirnac I.,
Gabbiani G.,
Lübbert H.
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb16687.x
Subject(s) - receptor , vascular smooth muscle , 5 ht receptor , forskolin , endocrinology , medicine , biology , ketanserin , receptor antagonist , microbiology and biotechnology , serotonin , antagonist , smooth muscle , biochemistry
Human uterine artery smooth muscle cells in culture were shown to express constitutively both 5‐ht 7 receptor mRNA and 5‐ht 7 ‐like receptors functionally linked to cyclic AMP formation. 5‐Carboxamidotryptamine (5‐CT) and 5‐HT enhanced forskolin‐stimulated cyclic AMP accumulation in these cells, with pEC 50 values of 7.12 and 6.25, sumatriptan being very weakly active. Both methiothepin (0.1 μ m ) and clozapine (1 μ m ), but not the 5‐HT 4 ‐receptor antagonist, SDZ 205–557 (10 μ m ) antagonized the effects of 5‐CT. In reverse transcriptase‐polymerase chain reaction analysis, the mRNA for 5‐ht 7 , but not for 5‐HT 4 or 5‐ht 6 receptors was found to be strongly expressed in the same cells. These findings represent a further step toward the recognition of 5‐ht 7 receptors as real, functional receptors.