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Interaction of nitric oxide synthase inhibitors and their D‐enantiomers with rat neutrophil luminol dependent chemiluminescence response
Author(s) -
Dikshit M.,
Chari S.S.,
Seth P.,
Kumari R.
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15711.x
Subject(s) - chemistry , nitric oxide , chemiluminescence , myeloperoxidase , luminol , nitric oxide synthase , biochemistry , lucigenin , nadph oxidase , pharmacology , enantiomer , arginine , n formylmethionine leucyl phenylalanine , superoxide , stereochemistry , reactive oxygen species , enzyme , biology , inflammation , immunology , chromatography , amino acid , organic chemistry
1 Formyl‐methionyl‐leucyl‐phenylalanine (FMLP) or arachidonic acid (AA) induced luminol dependent chemiluminescence (LCL) response of rat polymorphonuclear leukocytes (PMNLs) was found to be inhibited by nitric oxide synthease inhibitors and their D‐enantiomers. 2 Rat PMNLs LCL response was inhibited by N G ‐nitro‐L‐arginine methyl ester (L‐NAME), D‐NAME, N G ‐monomethyl‐L‐arginine (L‐NMMA) or D‐NMMA, in a concentration‐ and time‐dependent manner. 3 It was observed that both L‐ and D‐enantiomers of the arginine analogues (1000 μ m ) did not inhibit AA induced lucigenin‐dependent chemiluminescence (LUCDCL) response and cytochrome c reduction, used for estimating the NADPH‐oxidase activity in the cells and in the cell free system, respectively. 4 None of the L‐ and D‐enantiomers had any effect on either rat basal PMNLs or AA‐induced oxygen consumption. 5 In addition, neither the L nor D‐enantiomers of NAME altered either AA‐induced release or the activity of myeloperoxidase from rat PMNLs azurophilic granules. 6 The results obtained indicate that the attenuation of the LCL response by L‐ and D‐enantiomers of arginine analogues, is a non‐specific effect as there was no inhibition of NADPH‐oxidase and MPO activity, MPO release or oxygen consumption. Therefore, the data obtained indicate that these agents should be used with caution to analyse the role of nitric oxide in rat PMNLs LCL response.