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Sequential onset of three 5‐HT receptors during the 5‐hydroxytryptaminergic differentiation of the murine 1C11 cell line
Author(s) -
Kellermann Odile,
Loric Sylvain,
Maroteaux Luc,
Launay JeanMarie
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15519.x
Subject(s) - receptor , clone (java method) , 5 ht receptor , biology , cell culture , cellular differentiation , forskolin , microbiology and biotechnology , agonist , serotonin , medicine , endocrinology , gene , biochemistry , genetics
1 The murine 1C11 clone, which derives from a multipotential embryonal carcinoma cell line, has the features of a neuroectodermal precursor. When cultured in the presence of dibutyryl cyclic AMP, the 1C11 cells extend bipolar extensions and express neurone‐associated markers. After 4 days, the resulting cells have acquired the ability to synthesize, take up, store and catabolize 5‐hydroxytryptamine (5‐HT). We have thus investigated the presence of 5‐HT receptors during the 5‐hydroxytryptaminergic differentiation of this inducible 1C11 cell line. 2 As shown by the binding of [ 125 I]‐GTI and the CGS 12066‐dependent inhibition of the forskolin‐induced cyclic AMP production, functional 5‐HT 1B/1D receptors become expressed on day 2 of 1C11 cell differentiation. The density of these receptors remained unchanged until day 4. 3 The same holds true for the 5‐HT 2B receptor, also identified by its pharmacological profile and its positive coupling to the phosphoinositide cascade. 4 On day 4 of 1C11 cell differentiation, a third 5‐HT receptor, pharmacologically and functionally similar to 5‐HT 2A , had become induced. 5 Strikingly, the amounts of each transcript encoding 5‐HT 1B , 5‐HT 2A and 5‐HT 2B receptor did not vary significantly during the time course of the 1C11 5‐hydroxytryptaminergic differentiation. 6 The clone 1C11 may thus provide a useful in vitro model for studying regulation(s) between multiple G‐linked receptors as well as the possible role of 5‐HT upon the expression of a complete 5‐hydroxytryptamine phenotype.