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Chloride channel blockers decrease intracellular pH in cultured renal epithelial LLC‐PK 1 cells
Author(s) -
Brown Colin D.A.,
Dudley Adam J.
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15422.x
Subject(s) - intracellular , chloride , chemistry , pharmacology , endocrinology , biochemistry , biology , organic chemistry
The effects of chloride channel blockers upon intracellular pH (pH i ) were examined in renal epithelial monolayers of LLC‐PK 1 cells. A significant intracellular acidification was found with addition of 100 μ m 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoate (NPPB), niflumic acid, flufenamate and diphenylamine‐2‐carboxylate (DPC) but not with 4,4′‐diisothiocyanatostilbene‐2‐2′disulphonic acid (DIDS). The effects of these agents upon pH i was dose‐dependent with apparent K 0.5 values of: 16.7±0.3 μ m , 34.2±0.9 μ m and 740±13 μ m for niflumic acid, flufenamate and DPC respectively. The results indicate that at concentrations commonly used to block channel activity these chloride channel blockers have profound effects upon pH i .