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The effect of nociceptin on Ca 2+ channel current and intracellular Ca 2+ in the SH‐SY5Y human neuroblastoma cell line
Author(s) -
Connor Mark,
Yoe Alison,
Henderson Graeme
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15387.x
Subject(s) - nociceptin receptor , naltrindole , sh sy5y , pertussis toxin , endocrinology , chemistry , medicine , intracellular , antagonist , opioid receptor , endogenous opioid , neuroblastoma , opioid peptide , opioid , biophysics , receptor , cell culture , g protein , biology , biochemistry , genetics
The human neuroblastoma cell line SH‐SY5Y expresses the ‘orphan’ opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration‐dependent inhibition of the N‐type calcium channel current in these cells (IC 50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca 2+ (EC 50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 μ m ), naltrindole (1 μ m ) and naloxone (10 μ m ).