Premium
Inhibition of nitrergic relaxations by a selective inhibitor of the soluble guanylate cyclase
Author(s) -
Cellek Selim,
Kasakov Lubomir,
Moncada Salvador
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15376.x
Subject(s) - sodium nitroprusside , isoprenaline , chemistry , medicine , endocrinology , guanylate cyclase , stimulation , nitric oxide , cyclic gmp , atrial natriuretic peptide , snap , computer graphics (images) , computer science
1 . The actions of 1H‐[1,2,4]oxadiazolo[4,3,‐a]quinoxalin‐1‐one (ODQ), a specific inhibitor of the soluble guanylate cyclase (SGC), were investigated in the rabbit anococcygeus muscle. 2 . ODQ (1 nM‐1 μ m ) inhibited in a concentration‐dependent manner the relaxations induced by electrical field stimulation (EFS; 50 V, 0.3 ms duration, 1 Hz, for 5 s, every 120 s). 3 . ODQ (1 μ m ) also inhibited the relaxations elicited by EFS (50 V, 0.3 ms duration, 1, 2.5, 5, 10 Hz, for 5 s) and sodium nitroprusside (SNP; 1 μ m ) without affecting those induced by isoprenaline (1 μ m ), atrial natriuretic peptide (ANP; 100 nM) or an analogue of cyclic GMP (8‐pCPT‐cyclic GMP; 500 μ m ). 4 . ODQ (1 μ m ) inhibited the elevations in the concentration of cyclic GMP induced by SNP or EFS, but not by ANP. ODQ did not affect the concentrations of cyclic AMP. 5 . Nitrergic relaxation in this tissue appears, therefore, to be mediated via activation of SGC.