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In situ assessment of the role of the β 1 , β 2 ‐ and β 3 ‐adrenoceptors in the control of lipolysis and nutritive blood flow in human subcutaneous adipose tissue
Author(s) -
Barbe Pierre,
Millet Laurence,
Galitzky Jean,
Lafontan Max,
Berlan Michel
Publication year - 1996
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1996.tb15279.x
Subject(s) - isoprenaline , terbutaline , agonist , chemistry , lipolysis , endocrinology , dobutamine , medicine , adipose tissue , microdialysis , blood flow , perfusion , hemodynamics , stimulation , receptor , asthma , central nervous system
1 The involvment of β 1 , β 2 ‐ and β 3 ‐adrenoceptors in the control of lipolysis and nutritive blood flow was investigated in abdominal subcutaneous adipose tissue of healthy young adults by use of an in situ microdialysis technique. 2 Dialysis probes were infused either with isoprenaline (non‐selective β‐adrenoceptor agonist), CGP 12,177 (selective β 3 ‐adrenoceptor agonist having β 1 ‐/β 2 ‐antagonist properties), dobutamine (selective β 1 ‐adrenoceptor agonist) or terbutaline (selective β 2 ‐adrenoceptor agonist). The recovery of each probe used for perfusion was calculated by an in vivo calibration method. The local blood flow was estimated through the measurement of the escape of ethanol infused simultaneously with the drugs included in the probe. 3 Isoprenaline infusion at 0.01 μ m had a weak effect while higher concentrations of isoprenaline (0.1 and 1 μ m ) caused a rapid, sustained and concentration‐dependent increase of glycerol outflow; the maximum increase was 306±34% with 1 μ m . Isoprenaline also increased the nutritive blood flow in adipose tissue; a significant effect appeared at 0.1 μ m isoprenaline and was greater at 1 μ m . 4 CGP 12,177 (10 and 100 μ m ) increased the glycerol concentration in the dialysate (128±8 and 149±12%, respectively) and nutritive blood flow. Terbutaline and dobutamine (100 μ m ) both provoked rapid and similar increases in glycerol outflow (252±18 and 249±18%, respectively). Both, terbutaline and dobutamine increased nutritive blood flow. 5 It is concluded that β 1 and β 2 ‐adrenoceptor subtypes are both mainly involved in the mobilization of lipids and in the control of nutritive blood flow. β 3 ‐Adrenoceptors play a weaker role in the control of lipolysis and nutritive blood flow in human subcutaneous abdominal adipose tissue.