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Interaction between methotrexate and indomethacin on a human normal haemopoietic cell line
Author(s) -
Hollingsworth S.J.,
Shankley N.P.,
Anderson E.M.,
Bennett A.
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb17197.x
Subject(s) - methotrexate , lymphoblast , in vivo , microgram , pharmacology , in vitro , long term potentiation , cell culture , chemistry , drug interaction , cytotoxicity , medicine , biochemistry , drug , biology , receptor , genetics , microbiology and biotechnology
1 The interaction between methotrexate and indomethacin has been examined, at a physiological folate concentration (20 n m ), on a human normal lymphoblast‐like cell line (RPMI 1788) in vitro . 2 Indomethacin (1 μg ml −1 ) increased the reduction of lymphoblast growth caused by methotrexate (10–80 ng ml −1 ). 3 Indomethacin (0.1 and 1 μg ml −1 ) potentiated the cytotoxicity of methotrexate (20 and 40 ng ml −1 ) after 4 days in culture. 4 Indomethacin (0.4 μg ml −1 ) reduced the accumulation of tritium in lymphoblasts incubated with [ 3 H]‐methotrexate after 30 min; therefore initial drug accumulation was not responsible for the potentiation seen after 4 days. 5 If indomethacin increases the killing of human cancer cells by methotrexate in vivo , with a smaller potentiation on lymphoblasts, this combination may be beneficial in treating human malignancy.