Premium
Activation by intracellular GDP, metabolic inhibition and pinacidil of a glibenclamide‐sensitive K‐channel in smooth muscle cells of rat mesenteric artery
Author(s) -
Zhang H.,
Bolton T.B.
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb17190.x
Subject(s) - pinacidil , glibenclamide , intracellular , smooth muscle , chemistry , mesenteric arteries , cromakalim , medicine , endocrinology , pharmacology , artery , biochemistry , diabetes mellitus
1 Single‐channel recordings were made from cell‐attached and isolated patches, and whole‐cell currents zwere recorded under voltage clamp from single smooth muscle cells obtained by enzymic digestion of a small branch of the rat mesenteric artery. 2 In single voltage‐clamped cells 1 m m uridine diphosphate (UDP) or guanidine diphosphate (GDP) added to the pipette solution, or pinacidil (100 μ m ) a K‐channel opener (KCO) applied in the bathing solution, evoked an outward current of up to 100pA which was blocked by glibenclamide (10 μ m ). In single cells from which recordings were made by the ‘perforated patch’ (nystatin pipette) technique, metabolic inhibition by 1 m m NaCN and 10 m m 2‐deoxy‐glucose also evoked a similar glibenclamide‐sensitive current. 3 Single K‐channel activity was observed in cell‐attached patches only infrequently unless the metabolism of the cell was inhibited, whereupon channel activity blocked by glibenclamide was seen; pinacidil applied to the cell evoked similar glibenclamide‐sensitive channel activity. If the patch was pulled off the cell to form an isolated inside‐out patch, similar glibenclamide‐sensitive single‐channel currents were observed in the presence of UDP and/or pinacidil to those seen in cell‐attached mode; channel conductance was 20 pS (60:130 K‐gradient) and openings showed no voltage‐dependence and noisy inward currents, typical of the nucleoside diphosphate (NDP) activated K‐channel (K NDP ) seen previously in rabbit portal vein. 4 Formation of an isolated inside‐out patch into an ATP‐free solution did not increase the probability of channel opening which declined with time even when some single‐channel activity had occurred in the cell‐attached mode before detachment. However, application of 1 m m UDP or GDP, but not ATP, to inside‐out patches evoked single‐channel activity. Application of ATP‐free solution to isolated patches, previously exposed to ATP and in which channel activity had been seen, did not evoke channel activity. 5 It is concluded that small conductance K‐channels (K NDP ) open in smooth muscle cells from this small artery in response to UDP or GDP acting from the inside, or pinacidil acting from the outside; the same channels open during inhibition of metabolism presumably mainly due to the rise in nucleoside diphosphates, but a fall in the ATP concentration on the inside of the channel did not by itself evoke channel activity. Failure to respond to a fall in ATP concentration upon formation of an inside‐out patch could not be due to dephosphorylation of the channel because sometimes it had been active previously during cell‐attached recording. NDPs, instead of ATP, are more important regulators of K NDP channels. It is suggested that the K NDP is the main target K‐channel for KCOs.