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The effect of folate on the methotrexate/indomethacin interaction in a murine cancer cell line
Author(s) -
Hollingsworth S.J.,
Anderson E.M.,
Bennett A.
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb16667.x
Subject(s) - methotrexate , cytotoxicity , long term potentiation , chemistry , antifolate , cell culture , pharmacology , folate receptor , biochemistry , antimetabolite , in vitro , cancer cell , medicine , cancer , biology , receptor , genetics
1 The effect of folate on the interaction between methotrexate (a folate analogue) and indomethacin has been examined in murine NC carcinoma cells 2 Conditioning of NC cells to a physiological (20 nM) folate concentration after culture in a high folate concentration increased the response to methotrexate. The sensitivity of these conditioned cells to methotrexate related inversely to the folate concentration 3 At 20 nM and 2 μ m folate, indomethacin 1 μg ml −1 potentiated the cytotoxicity of methotrexate 4 and 8 ng ml −1 (both P<0.03) 4 When NC cells were incubated with [ 3 H]‐methotrexate at 20 nM and 2 μ m folate, there was a trend for increased tritium accumulation with indomethacin 0.36 μg ml −1 (1 μ m ; P<0.01) 5 We conclude that the folate concentration can affect the sensitivity of NC cells to methotrexate, although the degree of potentiation of cytotoxicity by indomethacin remains similar.