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The in vivo effect of lipopolysaccharide on the spontaneous release of transmitter from motor nerve terminals
Author(s) -
Liu Shing H.,
Sheu Tzong J.,
Lin Ruey H.,
LinShiau Shoei Y.
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb16659.x
Subject(s) - lipopolysaccharide , ouabain , in vivo , chemistry , phrenic nerve , pentoxifylline , nitric oxide , endocrinology , tumor necrosis factor alpha , medicine , polymyxin b , nitric oxide synthase , pharmacology , biology , biochemistry , antibiotics , respiratory system , sodium , microbiology and biotechnology , organic chemistry
1 , Jen Ai Road, 1st Section, Taipei, Taiwan. 1 The in vivo effect of E. coli lipopolysaccharide (LPS) on the spontaneous release of transmitter was studied in the isolated phrenic nerve‐diaphragm preparation of the mouse 2 The resting membrane potential was decreased and frequency of miniature endplate potentials (m.e.p.ps) was increased by treatment with LPS 3 Pretreatment of diaphragms with ouabain markedly increased the frequency of m.e.p.ps in control group but not in the LPS group 4 When mice were treated with polymyxin B (a LPS neutralizer), pentoxifylline (an inhibitor of tumour necrosis factor‐α formation) and N G ‐nitro‐L‐arginine (an inhibitor of nitric oxide (NO) synthase) the effects of LPS were reversed 5 These results suggest that LPS increases the spontaneous transmitter release through, at least in part, the pathways of tumour necrosis factor‐α and NO followed by an inhibition of the Na + ‐pump activity in the endplate area.