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Glucocorticoid‐induced annexin 1 secretion by monocytes and peritoneal leukocytes
Author(s) -
Coméra Christine,
RussoMarie Frarçise
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb15916.x
Subject(s) - secretion , brefeldin a , endocrinology , dexamethasone , glucocorticoid , medicine , nocodazole , annexin , biology , annexin a2 , monensin , chemistry , in vitro , microbiology and biotechnology , biochemistry , golgi apparatus , cell , endoplasmic reticulum , cytoskeleton
1 We have studied the ability of the glucocorticoid, dexamethasone, to induce annexin 1 secretion by either human blood monocytes or rat peritoneal leukocytes. 2 The in vivo treatment of rats with dexamethasone (1.25 mg kg −1 ) selectively induced secretion of annexin 1 by peritoneal leukocytes, as assessed by incubating these cells in culture medium. Annexin 1 secretion was also induced in human cultured monocytes, in vitro , by 10 −6 m dexamethasone. 3 Annexin 1 secretion was inhibited in the presence of 20 mM NH 4 C1 or by conducting the experiments at 18°C. In contrast, it was not inhibited by monensin, nocodazole or brefeldin A. 4 The time necessary for annexin 1 synthesis and secretion was less than 15 min. 5 These data indicate that glucocorticoids induce annexin 1 secretion by monocytes or peritoneal leukocytes. Because it is not inhibited by monensin, nocodazole or brefeldin A and it is rapid, annexin 1 secretion seems to occur by the secretory pathway similar to that used by several cytosolic proteins such as interleukin‐iβ.