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Potentiation by tumour necrosis factor‐α of calcium signals induced by bradykinin and carbachol in human tracheal smooth muscle cells
Author(s) -
Amrani Yassine,
Martinet Nadine,
Bronner Christian
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb14896.x
Subject(s) - carbachol , bradykinin , endocrinology , medicine , calcium , tumor necrosis factor alpha , receptor , chemistry , biology
The effect of tumour necrosis factor‐α (TNFα) on the increase in cytosolic free calcium ([Ca 2+ ]) i induced by carbachol and bradykinin (BK) was investigated in human tracheal smooth muscle cells in culture (TSMC). BK (10 −12 –10 −9 m ) and carbachol (10 −7 –10 −3 m ) produced a concentration‐dependent increase in [Ca 2+ ] i (pD 2 = 10.73 ± 0.05 and 5.57 ± 0.03 respectively). The increase in [Ca 2+ ] i was significantly enhanced for both agonists in TNFα‐treated cells (10 ng ml −1 for 24 h). However, pD 2 values were not modified (10.78 ± 0.03 and 5.62 ± 0.04 for BK and carbachol, respectively) suggesting that no change in the apparent receptor affinity occured. Thus, TNFα induced‐alterations in Ca 2+ homeostasis in human TSMC may be a key mechanism underlying airway hyperreactivity.