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Inhibition of serum‐induced proliferation of bovine tracheal smooth muscle cells in culture by heparin and related glycosaminoglycans
Author(s) -
Kilfeather S.A.,
Tagoe S.,
Perez A.C.,
OkonaMensa K.,
Matin R.,
Page C.P.
Publication year - 1995
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1995.tb13367.x
Subject(s) - glycosaminoglycan , heparin , cell division , disaccharide , chemistry , cell growth , biochemistry , sulfation , cell culture , heparan sulfate , microbiology and biotechnology , endocrinology , medicine , biology , cell , genetics
1 The effect of heparin and related glycosaminoglycans on bovine airway smooth muscle proliferation has been investigated. 2 Foetal bovine serum stimulated division of bovine trachealis smooth muscle cells in a concentration‐dependent fashion at concentrations between 1 and 30%. 3 Heparin (0.1–100 μg ml‐1), heparan sulphate (0.1–100 μg ml −1 ) and fragmin (0.1–100 μg ml −1 ) inhibited smooth muscle division in a concentration‐dependent fashion between 0.1–100 μg ml −1 . A heparin disaccharide did not exhibit inhibition of division at 100 μg ml −1 . 4 Dextran sulphate at molecular weights of 5 × 10 3 and 5 × 10 5 concentration‐dependently inhibited division between 0.1–100 μg ml −1 . Dextran without sulphation did not exhibit inhibition of division at 100 μg ml −1 . 5 The magnitude of inhibition of proliferation did not reach 100% for any compounds examined at concentrations up to 100 μg ml −1 during incubations for 5 and 14 days. IC 50 values for inhibition of proliferation ranged between 1–5 μg ml −1 . 6 These findings suggest that heparin and related glycosaminoglycans inhibit bovine airway smooth muscle cell division.