Premium
Inhibition by SK&F 96365 of Ca 2+ current, IL‐2 production and activation in T lymphocytes
Author(s) -
Chung S. Clare,
McDonald Thomas V.,
Gardner Phyllis
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb17072.x
Subject(s) - chemistry , current (fluid) , endocrinology , medicine , biology , physics , thermodynamics
1 By use of whole cell patch‐clamp and Indo‐1 fluorescence studies of the Jurkat T leukaemic cell line, we show that the new organic antagonist of receptor‐mediated Ca 2+ entry, SK&F 96365, inhibits the T cell Ca 2+ current in a dose‐dependent fashion, with an IC 50 of 12 μ m . 2 SK&F 96365 also inhibits [ 3 H]‐thymidine incorporation and interleukin‐2 (IL‐2) synthesis in peripheral blood lymphocytes. 3 SK&F 96365 has no effect on Ca 2+ stores release or K + channels. 4 This is the first account of an organic inhibitor of the T cell Ca 2+ current. The ability of SK&F 96365 to inhibit IL‐2 synthesis and cell proliferation suggests that a new class of related Ca 2+ channel blockers can be developed as immunosuppressive agents.