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Cyclothiazide unmasks AMPA‐evoked stimulation of [ 3 H]‐L‐glutamate release from rat hippocampal synaptosomes
Author(s) -
Barnes Janine M.,
Dev Kumlesh K.,
Henley Jeremy M.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb16902.x
Subject(s) - ampa receptor , cnqx , glutamate receptor , chemistry , stimulation , tetrodotoxin , neuroscience , kainate receptor , neurotransmitter , hippocampal formation , biophysics , pharmacology , biology , receptor , biochemistry
The effect of α‐amino‐3‐hydroxy‐5‐methylisoxazolepropionate (AMPA) on Ca 2+ ‐sensitive, tetrodotoxin (TTX)‐insensitive K + ‐stimulated [ 3 H]‐L‐glutamate release from rat hippocampal synaptosomes was determined. AMPA in the presence, but not in the absence of cyclothiazide, a drug which blocks AMPA receptor desensitization, elicited a dose‐dependent increase in K + ‐stimulated [ 3 H]‐L‐glutamate release but had no effect on basal release. The AMPA/cyclothiazide stimulation was blocked by CNQX and by GYKI 52466, an antagonist at the cyclothiazide site. These results indicate that AMPA receptors are present on presynaptic terminals and suggest that they may play a role in the regulation of neurotransmitter release.