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Effects of ethanol and other aliphatic alcohols on NO‐mediated relaxations in rat anococcygeus muscles and gastric fundus strips
Author(s) -
Rand M.J.,
Li C.G.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14856.x
Subject(s) - sodium nitroprusside , chemistry , stimulation , ethanol , acetaldehyde , endocrinology , medicine , biochemistry , nitric oxide , organic chemistry
1 In anococcygeus muscles, ethanol (20–500 m m ) slightly increased the tone and inhibited relaxations elicited by nitrergic nerve stimulation (0.5–5 Hz) in a concentration‐dependent manner. 2 Other aliphatic alcohols decreased the tone but had inhibitory effects similar to ethanol on stimulation‐induced relaxations, the EC 50 (m m ) values being: methanol 280, ethanol 80, propan‐1‐ol 20, propan‐2‐ol 55, propan 1,2‐diol 135, butan‐1‐ol 120, butan‐2‐ol 15 and pentan‐1‐ol 3. 3 Relaxations induced by sodium nitroprusside (SNP, 10 n m ) were inhibited by ethanol (20–500 m m ) in a concentration‐dependent manner and by propan‐2‐ol (100 m m ). Relaxations induced by NO (1 μ m ) were inhibited by high concentrations of ethanol (200–300 m m ) and by propan‐2‐ol (100 m m ). 4 In gastric fundus strips, ethanol (60–200 m m ) did not affect the resting tone but inhibited NO‐mediated relaxations elicited by low frequency (1 Hz) field stimulation and reduced the initial relaxation by high frequency field stimulation (10 Hz) and by SNP (50 n m ). The relaxant action of isoprenaline (10 n m ) was not reduced although it was slightly slower in onset. Other aliphatic alcohols tested decreased the tone and inhibited relaxations elicited by field stimulation. 5 Acetaldehyde (1–10 m m ) inhibited relaxations elicited by field stimulation and SNP in both the rat anococcygeus muscles and gastric fundus strips. The tone of gastric fundus strips was decreased by acetaldehyde but it was transiently increased in anococcygeus muscles. 6 The dehydrogenase inhibitors, 4‐methylpyrazole (100 μ m ) and disulfiram (100 μ m ) did not significantly affect the inhibition by ethanol of nitrergic nerve stimulation‐induced relaxations in anococcygeus muscles, which suggests that ethanol acts directly. 7 The inhibition of NO‐mediated relaxations by alcohols is attributed to sequestration of NO to form nitroso‐alcohols.