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Interleukin‐1β enhances capsaicin‐induced neurogenic vasodilatation in the rat skin
Author(s) -
Herbert M.K.,
Holzer P.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14791.x
Subject(s) - capsaicin , calcitonin gene related peptide , vasodilation , endocrinology , chemistry , medicine , proinflammatory cytokine , stimulation , sensitization , hyperaemia , pharmacology , axon reflex , subcutaneous injection , saline , anesthesia , neuropeptide , blood flow , inflammation , immunology , receptor
1 This study examined the effect of interleukin‐1β (IL‐1β) on the capsaicin‐induced increase in cutaneous blood flow of anaesthetized rats as measured by laser Doppler flowmetry. 2 The substances were administered by intraplantar subcutaneous injection of 10 μl‐volumes, saline being injected into one hindpaw and IL‐1β into the other. 3 IL‐1β (0.5–500 pg) was without effect on blood flow on its own but dose‐dependently enhanced the hyperaemic response to intraplantar capsaicin (0.3 μg) up to 180% ( P < 0.05) of the response seen in saline‐treated paws. 4 Il‐1β‐(163–171), a fragment devoid of proinflammatory activity, failed to enhance capsaicin‐induced hyperaemia when given at a dose of 50 pg. 5 Indomethacin (10 mg kg −1 , i.p.) did not alter the capsaicin‐induced vasodilatation but prevented IL‐1β (50 pg) from augmenting the hyperaemic response to capsaicin. 6 The hyperaemia evoked by intraplantar calcitonin gene‐related peptide (0.038–3.8 ng) was not altered by IL‐1β (50 pg). 7 These data indicate that IL‐1β enhances the cutaneous hyperaemic response to afferent nerve stimulation with capsaicin in a prostaglandin‐dependent manner. This proinflammatory action of the cytokine appears to arise from sensitization of afferent nerve endings.