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Induction of emesis in Suncus murinus by pyrogallol, a generator of free radicals
Author(s) -
Torii Yoshifumi,
Saito Hiroshi,
Matsuki Norio
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14753.x
Subject(s) - pyrogallol , tropisetron , suncus , chemistry , radical , pharmacology , vagotomy , antagonist , dimercaprol , anesthesia , medicine , endocrinology , biochemistry , receptor
1 We investigated whether or not pyrogallol, a generator of free radicals, is emetogenic in Suncus murinus , the house musk shrew. Pyrogallol (i.p.) caused dose‐dependent emesis in suncus with an ED 50 value of 77.3 mg kg −1 . At a dose of 128 mg kg −1 , all suncus vomited with mean latency of 18.8 ± 5.2 min and the number of vomiting episodes was 8.6 ± 2.9. 2 The prophylactic effects of N‐(2‐mercaptopropionyl)‐glycine (MPG), an antioxidant, and tropisetron, a 5‐hydroxytryptamine 3 (5‐HT 3 ) receptor antagonist, were studied. Pyrogallol (128 mg kg −1 , i.p.)‐induced emesis was prevented by treatment with MPG (i.p.) or tropisetron (s.c.) with ID 50 values of 149 mg kg −1 and 117 μg kg −1 , respectively. 3 Pyrogallol‐induced emesis was completely prevented by surgical abdominal vagotomy. 4 The present results indicate that pyrogallol‐induced emesis is characteristically very similar to that caused by cisplatin and support the idea that generation of free radicals causes the release of peripheral 5‐HT, which stimulates vagal afferent sensory nerves to cause emesis.