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Contribution of thromboxane A 2 to the antigen‐induced immediate asthmatic response mediated by IgG1 antibody by augmentation of bronchial responsiveness in guinea‐pigs
Author(s) -
Arimura Akinori,
Asanuma Fujio,
Kurosawa Atsushi,
Harada Minoru
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14065.x
Subject(s) - immunology , guinea pig , antigen , antibody , thromboxane a2 , medicine , thromboxane , ovalbumin , asthma , endocrinology , platelet
1 IgG1‐mediated anaphylactic bronchoconstriction was elicited by intravenous administration of antigen to guinea‐pig 2 days after passive sensitization with IgG1‐rich serum, and this response was not affected by heating the serum (at 56°C, for 4 h). IgE‐mediated bronchoconstriction, provoked 14 days after passive sensitization with IgE‐rich serum, was completely abolished by the heating of the serum. 2 S‐1452 (10 mg kg −1 , p.o.), a selective thromboxane (Tx) A 2 antagonist, significantly but incompletely suppressed the IgG1‐mediated bronchoconstriction, but did not affect the IgE‐mediated one, while diphenhydramine (5 mg kg −1 , i.v.), a histamine antagonist, almost completely inhibited both IgG1‐ and IgE‐mediated bronchoconstriction. 3 Pretreatment with propranolol (1 mg kg −1 , i.v.), a β‐adrenergic blocker, in addition to diphenhydramine, caused a long‐lasting bronchoconstriction following antigen challenge in both animal models. This histamine‐independent bronchoconstriction was markedly suppressed by S‐1452 at a low dose of 0.1 mg kg −1 . 4 A significant increase in bronchial responsiveness to i.v. acetylcholine (ACh), compared to the prechallenge value, occurred as early as 3 min and persisted for 24 h after antigen challenge in the IgG1 model, but was not observed in the IgE model. S‐1452 (10 mg kg −1 , p.o.) inhibited the IgG1‐mediated bronchial hyperresponsiveness, as assessed 60 min after antigen challenge. 5 A marked elevation of TxB 2 levels was observed in bronchoalveolar lavage fluid (BALF) 3 min after antigen challenge in the IgG1 model, while levels were not changed in the IgE model. In contrast, the plasma TxB 2 level assessed 1 min after antigen challenge was increased in both the IgG1 and IgE models. 6 The results indicate that the inhibition of IgG1‐ but not IgE‐mediated bronchoconstriction by higher doses of S‐1452 may result from the suppression of increased bronchial responsiveness to allergic mediators such as histamine, which is probably due to TxA 2 generated in the airway lumen rather than in plasma. In both the IgG1 and IgE models, plasma TxA 2 appeared to contribute directly to the bronchoconstriction, its action being almost completely masked by histamine‐mediated bronchoconstriction.