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Prevention by phosphodiesterase inhibitors of antigen‐induced contraction of guinea‐pig colonic smooth muscle
Author(s) -
Grous Marilyn,
Barnette Mary
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14053.x
Subject(s) - zaprinast , rolipram , ibmx , phosphodiesterase , guinea pig , contraction (grammar) , medicine , endocrinology , ec50 , ovalbumin , enzyme inhibitor , chemistry , phosphodiesterase inhibitor , muscle contraction , pharmacology , biology , enzyme , biochemistry , in vitro , forskolin , immunology , antigen , stimulation
1 The ability of various phosphodiesterase (PDE) inhibitors to reduce the initial and/or late response to ovalbumin (OVA) in isolated strips of guinea‐pig colonic smooth muscle from sensitized animals was examined. 2 Both the initial and late responses to OVA (0.05 mg ml −1 ) were inhibited by the non‐selective PDE inhibitor, 3‐isobutyl‐1‐methyl‐xanthine (IBMX, EC 50 = 26.0 and 6.1 μ m , respectively), and the selective inhibitors of low K m cyclic AMP specific PDE (PDE IV), (R)‐ and (S)‐ rolipram. The (S)‐ isomer (EC 50 = approximately 1.0 μ m for both responses) was about 10 fold less potent than the (R)‐ isomer (EC 50 = approximately 0.1 μ m for both responses). 3 Zaprinast, a selective inhibitor of the cyclic GMP‐specific PDE (PDE V) inhibited only the late response (EC 50 = 1.4 μ m ). 4 SK&F 94120, a selective inhibitor of the cyclic GMP‐inhibited low K m cyclic AMP PDE (PDE III), inhibited the initial response (45.9 ± 11.9%, P < 0.05) at the highest concentration tested (100 μ m ), and had no effect on the late response. 5 The results suggest that PDE inhibitors, especially PDE IV inhibitors, can attenuate the contractile response of guinea‐pig colon to OVA.