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Suramin and reactive blue 2 are antagonists for a newly identified purinoceptor on rat megakaryocyte
Author(s) -
Uneyama Hisayuki,
Uneyama Chikako,
Ebihara Satoru,
Akaike Norio
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14051.x
Subject(s) - suramin , purinergic receptor , p2y receptor , antagonist , chemistry , pharmacology , medicine , endocrinology , adenosine , receptor , biology , biochemistry
1 The effects of purinoceptor antagonists on ATP‐induced oscillatory K + ‐currents in rat isolated megakaryocytes were investigated. 2 Both reactive blue‐2 (RB‐2), a selective antagonist of the P 2Y purinoceptor, at concentrations of 0.3–10 μ m and suramin, a non‐selective P 2 purinoceptor antagonist, at 1–30 μ m blocked the ATP‐induced oscillation in a concentration‐dependent manner. 3 RB‐2 and suramin also blocked the ADP‐induced K + ‐current oscillation at the same concentration range as in the case of ATP. However, both suramin and RB‐2 had no effect on thrombin‐ and inositol 1,4,5‐trisphosphate (IP 3 )‐induced K + current oscillation, indicating that they act as specific purinoceptor antagonists. 4 Thus, the purinoceptors on megakaryocytes show the properties of the P 2 subtype according to their blockade by antagonists.