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Contractile properties of synthetic cationic polypeptides in guinea‐pig isolated trachea
Author(s) -
Spina D.,
Goldie R.G.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb14019.x
Subject(s) - guinea pig , arginine , mepyramine , chemistry , histamine , biochemistry , biology , endocrinology , antagonist , receptor , amino acid
1 The synthetic polypeptides, poly‐ l ‐arginine, poly‐ l ‐lysine and poly‐ d ‐lysine contract guinea‐pig isolated trachea in a concentration‐dependent, epithelium‐independent manner. Indomethacin augmented the contractile response to poly‐ l ‐arginine. 2 The contractile response to poly‐ l ‐arginine was not significantly inhibited by nicardipine, a selective l ‐type calcium channel blocker or by the histamine H 1 ‐receptor antagonist, mepyramine nor significantly augmented by the neutral endopeptidase inhibitor, phosphoramidon. 3 The contractile response to poly‐ l ‐arginine was inhibited in a concentration‐dependent manner by prior incubation of guinea‐pig tracheal rings with a number of anionic polypeptides including, low molecular weight heparin, poly‐ l ‐aspartic acid and bovine serum albumin. 4 In vitro capsaicin‐induced desensitization failed to attenuate the contractile response to poly‐ l ‐arginine, suggesting little, if any role for sensory neuropeptides in the functional response in the guinea‐pig. 5 Synthetic polypeptides induce an epithelium‐independent, charge‐dependent contraction of guinea‐pig isolated trachea.