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Expression of inducible nitric oxide synthase by neurones following exposure to endotoxin and cytokine
Author(s) -
MincGolomb Dahlia,
Tsarfaty Ilan,
Schwartz Joan P.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb13136.x
Subject(s) - nitric oxide synthase , microglia , neurotoxicity , mediator , nitric oxide , microbiology and biotechnology , lipopolysaccharide , biology , cytokine , inflammation , chemistry , immunology , endocrinology , toxicity , organic chemistry
In the CNS, nitric oxide (NO) has been implicated as both a mediator of neurotoxicity and a neuromodulator. The inducible NO synthase (iNOS), thought to mediate toxic effects of NO, has been attributed to glial cells in the CNS. We now report that cerebellar granule cell neurones can be stimulated by lipopolysaccharide and interferon‐γ to express iNOS in vitro , as demonstrated by reverse transcription‐polymerase chain reaction and fluorescent in situ hybridisation. The expression of both constitutive NO synthase (cNOS) and iNOS by neurones suggests that NO has diverse functions in the brain, and supports the possibility that iNOS plays a role in neuronal damage and inflammation following activation of brain microglia and production of cytokines.