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Stimulation by menthol of Cl secretion via a Ca 2+ ‐dependent mechanism in canine airway epithelium
Author(s) -
Chiyotani A.,
Tamaoki J.,
Takeuchi S.,
Kondo M.,
Isono K.,
Konno K.
Publication year - 1994
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1994.tb13112.x
Subject(s) - amiloride , chemistry , medicine , ussing chamber , endocrinology , respiratory epithelium , adenosine , epithelium , paracellular transport , ion transporter , stimulation , menthol , transepithelial potential difference , biochemistry , respiratory system , in vitro , sodium , permeability (electromagnetism) , organic chemistry , membrane , pathology
1 To investigate the effect of menthol on airway epithelial ion transport function, we studied the bioelectrical properties of canine cultured tracheal epithelium by Ussing's short‐circuit technique in vitro.2 Addition of menthol (10 −3 m ) to the mucosal but not the submucosal solution increased the short‐circuit current ( I sc ) from 6.2 ± 0.9 to 14.0 ± 2.2 μA cm −2 ( P < 0.001), and this effect was accompanied by increases in transepithelial potential difference and conductance. The response was dose‐dependent, with the maximal increase from the baseline value and the concentration required to produce a half‐maximal effect (EC 50 ) being 6.4 ± 0.9 μA cm −2 ( P < 0.001) and 40 μ m , respectively. 3 Other cyclic alcohols, including menthone and cyclohexanol, had no effect on the electrical properties. 4 The menthol‐induced increase in I sc was not altered by pretreatment of the cells with amiloride, indomethacin, or propranolol but was abolished by diphenylamine‐2‐carboxylate, furosemide or substitution of Cl with iodide in the medium. 5 Menthol (10 −2 m ) increased cytosolic levels of free calcium ([Ca 2+ ]i) from 98 ± 12 to 340 ± 49 n m ( P < 0.01) in fura‐2‐loaded tracheal epithelium but did not affect the intracellular adenosine 3′,5′‐cyclic monophosphate content. 6 These results suggest that menthol stimulates Cl secretion across airway epithelium, probably through a Ca 2+ ‐dependent mechanism, and might thus influence mucociliary transport in the respiratory tract.