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The action of Hoe 140 on the bradykinin‐induced splenic pressor reflex of the anaesthetized cat
Author(s) -
Louttit J.B.,
Coleman R.A.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13962.x
Subject(s) - ed50 , bradykinin , chemistry , antagonist , potency , pharmacology , endocrinology , medicine , receptor , in vitro , biochemistry
1 Intrasplenic injection of bradykinin (BK) induced a dose‐dependent pressor response in the anaesthetized cat, with an ED 50 of 0.98 ± 0.43 nmol. In contrast, intrasplenic administration of desArg 9 bradykinin (desArg 9 K) was without significant effect at doses of up to 200 nmol. 2 Intravenously administered BK induced a dose‐dependent depressor response in the anaesthetized cat, with an ED 50 of 0.86 ± 0.09 nmol kg −1 . desArg 9 BK was again without significant effect in this system at doses of up to 200 nmol kg −1 . 3 Both the pressor and depressor responses to BK were antagonized to a similar degree in a dose‐dependent manner by Hoe 140 (10 and 100 nmol kg −1 , i.v.). 4 At these doses, Hoe 140 appears to be a specific BK antagonist as it was ineffective against pressor responses to intrasplenic injection of capsaicin (5 nmol), while blocking those to an approximately equieffective dose of BK (1 nmol) in a dose‐dependent fashion. 5 Both the pressor response to intrasplenic BK and the depressor response to intravenous BK in the anaesthetized cat appear to be mediated by B 2 ‐receptors. This model may be useful in the quantitative determination of the antinociceptive potency of BK antagonists.