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PAF activation of a voltage‐gated R‐type Ca 2+ channel in human and canine aortic endothelial cells
Author(s) -
Bkaily Ghassan,
D'OrléansJuste Pedro,
Naik Radha,
Pérodin Jacqueline,
Stankova Jana,
Abdulnour Elias,
RolaPleszczynski Marek
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13841.x
Subject(s) - depolarization , nifedipine , antagonist , chemistry , biophysics , receptor , voltage dependent calcium channel , medicine , endocrinology , calcium , biology , biochemistry
By the use of fura‐2 and digital imaging techniques, [K] o depolarization or PAF (10 −9 m ) were shown to induce a sustained increase of [Ca] i in human or canine single aortic vascular endothelial cells (VEC) that was insensitive to nifedipine but sensitive to (−)‐PN200–110 or to lowering of [Ca] o . The PAF‐induced effect on [Ca] i was blocked by the PAF receptor antagonist, WEB2170. Our results suggest that [K] o depolarization and PAF increase [Ca] i via the activation of R‐type Ca 2+ channels.