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Effect of nafamostat mesilate on Na + and K + transport properties in the rabbit cortical collecting duct
Author(s) -
Muto Shigeaki,
Imai Masashi,
Asano Yasushi
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13626.x
Subject(s) - amiloride , apical membrane , chemistry , biophysics , membrane , epithelial polarity , conductance , ion transporter , membrane potential , cell membrane , membrane transport , intercalated cell , sodium , cell , biochemistry , biology , mathematics , organic chemistry , combinatorics
1 To determine the mechanism(s) of hyperkalemia caused by nafamostat mesilate (NM), a serine‐protease inhibitor, we investigated the effects of the drug on Na + and K + transport properties of the collecting duct (CD) cell in the isolated and perfused cortical collecting duct from rabbit kidneys. 2 NM at 10 −4 m in the lumen, hyperpolarized the apical membrane in parallel with increases in transepithelial resistance (R T ) and fractional apical membrane resistance ( f R A ). 3 These effects were completely inhibited by pretreatment with 50 μ m luminal amiloride, whereas they were not affected by luminal addition of 2 m m Ba 2+ . 4 NM at 10 −4 m in the bath slightly but significantly depolarized the basolateral membrane without any changes in R T or f R A , although NM at 10 −5 m in the bath had no effect on the electrical parameters. 5 It is concluded that NM mainly acts on the apical membrane of the CD cell and inhibits the amiloride‐sensitive Na + conductance in the apical membrane.