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Mg 2+ and caffeine‐induced intracellular Ca 2+ release in human vascular endothelial cells
Author(s) -
Zhang Aimin,
Cheng Toni P.O.,
Altura Bella T.,
Altura Burton M.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13568.x
Subject(s) - caffeine , intracellular , calcium , fura 2 , chemistry , biophysics , endothelial stem cell , magnesium , basal (medicine) , calcium metabolism , endocrinology , medicine , biochemistry , biology , in vitro , cytosol , enzyme , organic chemistry , insulin
Interaction of ionized magnesium ([Mg 2+ ] o ) and caffeine in regulation of intracellular free calcium concentration ([Ca 2+ ] i ) in human aortic endothelial cells was studied using fura‐2 and digital imaging microscopy. In 1.2 m m [Mg 2+ ] o , basal [Ca 2+ ] i was 73.7 ± 22.4 n m , with a heterogeneous distribution within the cells. No significant changes of basal [Ca 2+ ] i were found either when cells were treated with 10 m m caffeine or when [Mg 2+ ] o was lowered from 1.2 m m to 0.3 m m . However, a combined superfusion of the cells with 0.3 m m [Mg 2+ ] o and 10 m m caffeine resulted in a significant elevation of [Ca 2+ ] i to 382.8 ± 57.1 n m , probably by release of Ca 2+ from internal stores, which was attenuated by NiCl 2 (1 m m ). These results suggest that a Ca 2+ ‐induced Ca 2+ release mechanism is involved in regulation of [Ca 2+ ] i in endothelial cells, which may be either regulated or modulated by Mg 2+ .