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Influence of rhein anthrone on peristaltic reflex of guinea‐pig isolated ileum: involvement of prostaglandins
Author(s) -
Nijs Geert,
Witte Peter,
Geboes Karel,
Meulemans Ann,
Schuurkes Jan,
Lemli Joseph
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13473.x
Subject(s) - anthrone , chemistry , peristalsis , arachidonic acid , guinea pig , ileum , prostaglandin , prostaglandin e2 , prostaglandins f , endocrinology , medicine , chromatography , biochemistry , enzyme
1 The influence of rhein anthrone on the peristaltic reflex was studied with a modified Trendelenburg technique in a range from 10 −8 m to 4 × 10 −5 m , on a normal and reversed guinea‐pig ileum segment. Rhein anthrone had no significant effects on longitudinal muscle tension, intraluminal pressure or volume displacement when tested on the normal segment in doses up to 10 −5 m . When applied to the mucosal side (reversed segment), rhein anthrone produced a dose‐dependent increase of longitudinal muscle tension (significant from 10 −7 m ), of intraluminal pressure (significant from 3 × 10 −6 m ) and of volume displacement (significant from 10 −7 m ). The data show that rhein anthrone possesses in vitro activity which is dependent on contact with the mucosa. 2 The action of rhein anthrone on the reversed segment was inhibited by BW755C (a dual inhibitor of cyclo‐oxygenase and lipoxygenase), by indomethacin and by SC19220 (an antagonist of prostaglandin E 2 (PGE 2 ) and PGF 2α ). The effects remaining on longitudinal muscle tension, intraluminal pressure and volume displacement, calculated as percentage (mean ± s.e.mean) of the initial value, were respectively: 13 ± 8; 23 ± 13; 112 ± 5 for BW755C; 66 ± 19; 51 ± 8; 53 ± 8 for indomethacin and 27 ± 12; 13 ± 7; 50 ± 5 for SC19220. It is concluded that arachidonic acid metabolites, especially PGE 2 and PGF 2α are involved in the effects of rhein anthrone on the reversed segment.