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A factor released by monocytes in the presence of dexamethasone stimulates neutrophil locomotion
Author(s) -
Chettibi S.,
Lawrence A.J.,
Stevenson R.D.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13470.x
Subject(s) - monocyte , dexamethasone , antiserum , peripheral blood mononuclear cell , polyclonal antibodies , steroid , endocrinology , medicine , in vivo , chemistry , in vitro , immunology , biology , antibody , biochemistry , hormone , microbiology and biotechnology
1 Steroid‐treated monocyte supernatants cause a dramatic increase in the speed of locomotion of human neutrophils and a significant decrease in their adhesion to protein‐coated glass. In contrast, control monocyte supernatants have a smaller effect on the speed of locomotion, but cause a large increase in their adhesiveness. 2 This supernatant activity was produced equally well in the presence or absence of serum after 24 h culture at 37°C with 10 −6 m dexamethasone. 3 The effect of the steroid‐treated monocyte supernatants on the speed of locomotion of human peripheral blood neutrophils was not altered by rabbit polyclonal antisera against lipocortins 1–6. 4 Rabbit anti‐interleukin‐8 antibody which blocked the effect of IL‐8 on the speed of locomotion of neutrophils did not antagonize the locomotion stimulating action of steroid‐treated monocyte supernatants. 5 The exocellular release of this factor(s) by human mononuclear leucocytes suggests that it may be an in vivo mediator of the anti‐inflammatory effect of glucocorticoids.