Premium
Nitric oxide mediates the inhibition by interleukin‐1β of pentagastrin‐stimulated rat gastric acid secretion
Author(s) -
Esplugues Juan V.,
Barrachina M. Dolores,
Calatayud Sara,
Pique Josep M.,
Whittle Brendan J.R.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb13431.x
Subject(s) - pentagastrin , endocrinology , medicine , gastric acid , bolus (digestion) , nitric oxide , chemistry , arginine , phenylephrine , stomach , blood pressure , biochemistry , amino acid
Bolus injection of interleukin‐1β (2 μg kg −1 , i.v.) inhibited acid secretion induced by intravenous infusion of pentagastrin (8 μg kg −1 h −1 ) in the continuously perfused stomach of the anaesthetized rat. Administration of interleukin‐1β did not modify mean systemic arterial blood pressure. Pretreatment with N G ‐nitro‐ l ‐arginine methyl ester ( l ‐NAME, 2 −10 mg kg −1 , i.v.), but not dexamethasone (5 mg kg −1 , s.c. twice over 16 h), restored the acid secretory responses to pentagastrin. The actions of l ‐NAME were reversed by the prior administration of l ‐arginine (100 mg kg −1 , i.v.), but not by its enantiomer d ‐arginine (100 mg kg −1 , i.v.). l ‐NAME (5 mg kg −1 , i.v.) increased blood pressure but this was not the mechanism by which interleukin‐induced acid inhibition was prevented, since similar systemic pressor responses induced by phenylephrine (10 μg kg −1 min −1 , i.v.), had no such effect. These findings suggest that interleukin‐induced inhibition of acid responses to pentagastrin involves synthesis of NO from l ‐arginine.