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The modulation of inflammatory oedema by calcitonin gene‐related peptide
Author(s) -
Newbold P.,
Brain S.D.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb12865.x
Subject(s) - calcitonin gene related peptide , endocrinology , medicine , substance p , capsaicin , calcitonin , chemistry , prostaglandin , neurogenic inflammation , neuropeptide , vasodilation , pharmacology , receptor
1 The effect of calcitonin gene‐related peptide (CGRP) when given with or as a pretreatment to oedema inducing agents was investigated in the skin and paws of male Wistar and Sprague Dawley rats. 2 Oedema formation at intradermally‐injected sites in the skin was measured by a 125 I‐labelled human serum albumin accumulation technique and paw oedema was measured by a weight displacement technique. 3 CGRP (30 pmol) when given with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by substance P (100 pmol) in rat skin. In comparison, CGRP had little effect on 5‐hydroxytryptamine (5‐HT, 0.1–3 nmol)‐induced oedema when given as a co‐injection but significantly potentiated 5‐HT‐induced oedema when given as a 20 min pretreatment in the skin. Similar results were obtained in both Wistar and Sprague Dawley rats. 4 Pretreatment with CGRP (30 pmol) had little modulatory effect on oedema induced by substance P (100 pmol) in the presence of the vasodilator prostanoid, prostaglandin E 1 (PGE 1 , 850 pmol) in the skin of Wistar rats. 5 Pretreatment with CGRP (30 pmol) caused a non‐significant increase in carrageenin (300 μg)‐induced oedema in the hind paw of Wistar rats. Capsaicin (100 nmol) given as a pretreatment had little effect on carrageenin‐induced oedema. 6 CGRP (30 pmol), given as a pretreatment, had little modulatory effect on 5‐HT (3 nmol)‐induced oedema in the paw of Wistar rats but a non‐significant decrease in paw oedema was observed in Sprague Dawley rats. 7 The results suggest that CGRP, either given together with or as a pretreatment to mediators of increased microvascular permeability, depending on circumstance, can act in a synergistic manner to increase oedema formation. Little evidence was obtained for an anti‐inflammatory effect of CGRP in either the rat skin or paw.