Premium
Activation of ion channels by lysylbradykinin in the HCA‐7 colony 29 human adenocarcinoma cell line
Author(s) -
Henderson R.M.,
Cuthbert A.W.
Publication year - 1993
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1993.tb12828.x
Subject(s) - ion channel , cytosol , patch clamp , biophysics , membrane potential , hyperpolarization (physics) , chemistry , voltage gated ion channel , secretion , cell , membrane , microbiology and biotechnology , electrophysiology , biology , biochemistry , neuroscience , stereochemistry , receptor , enzyme , nuclear magnetic resonance spectroscopy
1 The patch‐clamp technique, both cell attached and inside‐out patches, was used to examine the effects of lysylbradykinin (LBK) and A23187 on ion channels in cultured Colony 29 epithelial cells derived from a human adenocarcinoma. 2 LBK and A23187 applied directly to the intact cell stimulated the opening of a number of types of ion channel including Ca 2+ ‐activated K + channels. 3 By use of inside‐out patches, anion channels could be stimulated to open by application of protein kinase A and ATP to the cytosolic surface. Ca 2+ ‐activated K + channels were also identified in isolated membrane patches. 4 The results suggest that the anion secretion which is stimulated by LBK is a complex event, involving the activation of a number of different types of ion channel, and that part of the response is the result of hyperpolarization of the cell by activation of Ca 2+ ‐activated K + channels. From the data presented in this and the accompanying papers it appears that the Ca 2+ ‐sensitive K + channels would be equally effective in either the apical or basolateral membranes.