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ATP mediates excitatory synaptic transmission in mammalian neurones
Author(s) -
Silinsky E.M.,
Gerzanich V.,
Vanner S.M.
Publication year - 1992
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1992.tb14408.x
Subject(s) - excitatory postsynaptic potential , suramin , neurotransmission , acetylcholine , adenosine triphosphate , neurotransmitter , biophysics , adenosine , chemistry , biology , postsynaptic current , neuroscience , inhibitory postsynaptic potential , biochemistry , endocrinology , in vitro , central nervous system , receptor
Adenosine 5′‐triphosphate (ATP, 0.1–100 μ m ), produced inward currents in patch‐clamped coeliac neurones from guinea‐pig when studied in either the whole cell configuration or in excised (outside‐out) patches. The P 2 ‐purinoceptor antagonists suramin (80–230 μ m ) or reactive blue 2 (2–20 μ m ) depressed the ATP‐induced currents but not those produced by acetylcholine. Excitatory post‐synaptic currents (e.p.s.cs) were observed in cultured neurones. E.p.s.cs had similar current‐voltage relationships to currents evoked by ATP in excised patches and were reduced by suramin or reactive blue 2 to a similar extent as ATP currents. The results suggest that ATP is the excitatory neurotransmitter in cultures of these neurones.