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Characterization of nitric oxide synthases in non‐adrenergic non‐cholinergic nerve containing tissue from the rat anococcygeus muscle
Author(s) -
Mitchell Jane A.,
Sheng Hong,
Förstermann Ulrich,
Murad Ferid
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12422.x
Subject(s) - nitric oxide synthase , tetrahydrobiopterin , cholinergic , nitric oxide , chemistry , cytosol , biochemistry , atp synthase , arginine , adrenergic , vas deferens , citrulline , endocrinology , medicine , enzyme , biology , amino acid , receptor
Tissue homogenates prepared from rat anococcygeus muscle converted l ‐arginine to l ‐citrulline indicating the presence of nitric oxide (NO) synthase. NO synthase activity was also found in crude and partially‐purified soluble and particulate fractions prepared from the homogenates. Both soluble and particulate NO synthase were dependent on NADPH, 5,6,7,8‐tetrahydrobiopterin and calcium, and inhibited by N G ‐nitro‐ l ‐arginine. Tissue homogenates or crude cytosolic and membrane fractions from rat vas deferens, which does not contain NO releasing non‐adrenergic non‐cholinergic neurones, had no NO synthase activity.