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Nitroglycerin relaxes coronary artery of the pig with no change in glutathione content or glutathione S‐transferase activity
Author(s) -
Sakanashi Matao,
Matsuzaki Toshihiro,
Aniya Yoko
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12350.x
Subject(s) - glutathione , chemistry , medicine , endocrinology , artery , anesthesia , biochemistry , enzyme
1 The role of glutathione content and glutathione S‐transferase activity in vascular relaxant responses to nitroglycerin was evaluated in potassium (30 m m )‐contracted coronary artery strips of the pig by measuring changes in tension, glutathione content and glutathione S‐transferase activity. 2 Prior exposure of coronary artery strips to nitroglycerin (10 −5 m or 10 −4 m for 20 min) resulted in tachyphylaxis to subsequent relaxation to nitroglycerin (10 −8 –10 −5 m ). 3 The glutathione content and glutathione S‐transferase activity of the arterial strips rendered tachyphylactic by prior exposure to nitroglycerin (10 −5 m for 20 min or 10 −3 m for 120 min) were not significantly different from those of control strips. 4 Treatment with diethyl maleate (10 −4 m or 10 −3 m for 60 min) markedly depleted arterial glutathione content in a concentration‐dependent manner with no change in glutathione S‐transferase activity. 5 The relaxant response of coronary artery strips to nitroglycerin (10 −8 –10 −5 m ) was completely unaffected following treatment with diethyl maleate (10 −4 m or 10 −3 m for 60 min). 6 The results suggest that vascular glutathione content does not play an important role in vascular relaxation or tolerance development to nitroglycerin, at least in pig isolated coronary artery.