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Antagonism by reactive blue 2 but not by brilliant blue G of extracellular ATP‐evoked responses in PC12 phaeochromocytoma cells
Author(s) -
Inoue Kazuhide,
Nakazawa Ken,
OharaImaizumi Mica,
Obama Tomoko,
Fujimori Kannosuke,
Takanaka Akira
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12265.x
Subject(s) - extracellular , purinergic receptor , antagonism , antagonist , chemistry , secretion , biophysics , endocrinology , medicine , biology , biochemistry , receptor
1 The effects of reactive blue 2 and brilliant blue G, which have been shown to block extracellular ATP‐evoked responses, were investigated to discover whether these compounds act as P 2 ‐purinoceptor antagonists in PC12 phaeochromocytoma cells. 2 Reactive blue 2 (10 to 100 μ m ) suppressed the ATP‐stimulated dopamine secretion from PC12 cells in a dose‐dependent manner. The concentration‐response curve for ATP was shifted to the right and the maximal response was decreased by reactive blue (30 and 100 μ m ). Brilliant blue G (up to 100 μ m ) did not significantly affect the secretion. 3 Reactive blue 2 (10 to 100 μ m ) suppressed the ATP‐activated inward current recorded from the voltage‐clamped cells in a concentration‐dependent manner. Brilliant blue G (up to 100 μ m ) did not affect the current. 4 The results suggest that reactive blue 2 but not brilliant blue G is a P 2 ‐purinoceptor antagonist in PC12 cells. The purinoceptors in these cells may be the same type as those involved in ATP‐evoked smooth muscle relaxation, judging from the antagonism by reactive blue 2.