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Characterization and actions of human umbilical endothelium derived relaxing factor
Author(s) -
Chaudhuri G.,
Buga Georgette M.,
Gold Michele E.,
Wood Keith S.,
Ignarro Louis J.
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12174.x
Subject(s) - endothelium derived relaxing factor , umbilical vein , bradykinin , acetylcholine , medicine , endocrinology , chemistry , anatomy , biochemistry , in vitro , receptor
1 A bioassay cascade superfusion technique was utilized to study the properties of endothelium derived relaxing factor (EDRF) from human umbilical vein (HUV) and compare its actions on umbilical, chorionic plate and bovine pulmonary arterial strips. 2 Histamine (1 μ m ), bradykinin (1 μ m ) and A‐23187 (0.3 μ m , 1 μ m ) but not acetylcholine (1 μ m ) released EDRF. 3 The non‐innervated human foetoplacental vessels, i.e., umbilical and chorionic plate arteries, do relax to EDRF by a guanosine 3′: 5′‐cyclic monophosphate (cyclic GMP)‐mediated mechanism. 4 The sensitivity of the human umbilical arterial strips to EDRF was less than that of the chorionic plate arterial strips. Bovine pulmonary arterial strips were the most sensitive to the relaxant actions of human umbilical EDRF.