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Properties of 5‐hydroxytryptamine 3 receptor‐gated currents in adult rat dorsal root ganglion neurones
Author(s) -
Robertson Brian,
Bevan Stuart
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12165.x
Subject(s) - methysergide , dorsal root ganglion , extracellular , divalent , depolarization , medicine , endocrinology , chemistry , calcium , biophysics , staurosporine , antagonist , receptor , biology , anatomy , protein kinase a , biochemistry , kinase , dorsum , organic chemistry
1 Responses to 5‐hydroxytryptamine (5‐HT) were examined on rat dorsal root ganglion (DRG) neurones maintained in tissue cultures, by use of whole cell recording techniques. 2 5‐HT (usually 10 μ m ) evoked a depolarization associated with an increase in membrane conductance in 40% of DRG neurones. There was a considerable variation in the size and persistence of this response between different batches of cells. 3 The 5‐HT response was mimicked by applying the agonists 2‐methyl‐5‐HT (10 μ m ) and phenylbiguanide (10 μ m ). Responses were blocked by ICS 205–930 (100 n m ), but not by methysergide (0.1–1.0 μ m ). 4 5‐HT currents could be carried by sodium and caesium ions, but not by choline ions. The amplitude and duration of the 5‐HT responses were dependent on the concentration of divalent cations in the extracellular solution: both became greater when calcium and magnesium concentrations were decreased. 5 Staurosporine, a putative antagonist of protein kinases, inhibited responses to 5‐HT.