Premium
Presynaptic inhibition by neuropeptide Y in rat hippocampal slice in vitro is mediated by a Y 2 receptor
Author(s) -
Colmers William F.,
Klapstein Gloria J.,
Fournier Alain,
StPierre Serge,
Treherne Kerri A.
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12129.x
Subject(s) - postsynaptic potential , neuropeptide y receptor , excitatory postsynaptic potential , neurotransmission , agonist , hippocampal formation , extracellular , chemistry , biology , receptor , inhibitory postsynaptic potential , medicine , neuroscience , endocrinology , neuropeptide , microbiology and biotechnology , biochemistry
1 The action of analogues and C‐terminal fragments of neuropeptide Y (NPY) was examined on excitatory synaptic transmission in area CA1 of the rat hippocampal slice in vitro , by use of intracellular and extracellular recordings, to determine by agonist profile the NPY receptor subtype mediating presynaptic inhibition. 2 Neither NPY, analogues nor fragments of NPY affected the passive or active properties of the postsynaptic CA1 pyramidal neurones, indicating their action is at a presynaptic site. 3 The full‐sequence analogues, peptide YY (PYY) and human NPY (hNPY), were equipotent with NPY at the presynaptic receptor, while desamido hNPY was without activity. 4 NPY2–36 was equipotent with NPY. Fragments as short as NPY13–36 were active, but gradually lost activity with decreasing length. NPY16–36 had no effect on extracellular field potentials, but still significantly inhibited excitatory postsynaptic potential amplitudes. Fragments shorter than NPY16–36 had no measurable effect on synaptic transmission. 5 The presynaptic NPY receptor in hippocampal CA1 therefore shares an identical agonist profile with the presynaptic Y 2 receptor at the peripheral sympathetic neuroeffector junction.