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Bicuculline blocks nicotinic acetylcholine response in isolated intermediate lobe cells of the pig
Author(s) -
Zhang ZhongWei,
Feltz Paul
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb12125.x
Subject(s) - acetylcholine , bicuculline , nicotinic agonist , chemistry , neuroscience , pharmacology , biology , biochemistry , antagonist , receptor
1 The effect of bicuculline on nicotinic acetylcholine (ACh) responses in isolated intermediate lobe (IL) cells of the pig was investigated by use of patch‐clamp techniques. Bicuculline was found to reduce AChevoked whole‐cell currents (I ACh ) in all cells tested ( n = 40). 2 The blocking effect of bicuculline on I ACh was dose‐dependent, the concentration producing half‐maximal blockade being 43.8 μ m . 3 The blockade of I ACh by bicuculline was not voltage‐dependent at membrane potentials above −60 mV, but a slight voltage‐dependence was observed at holding potentials (HP) of −80 and −100 mV. 4 The inhibitory effect of bicuculline on I ACh was partially competitive at a HP of −60 mV. 5 Neither SR 95531, a pyridazinyl γ‐aminobutyric acid derivative, nor t ‐butylbicyclophosphorothionate (TBPS) blocked I ACh in IL cells. 6 It is concluded that bicuculline interacts directly with the ACh receptor‐ionophore complex on porcine IL cells.