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Neuropeptide Y inhibits Ca 2 + influx into cultured dorsal root ganglion neurones of the rat via a Y 2 receptor
Author(s) -
Bleakman D.,
Colmers W.F.,
Fournier A.,
Miller R.J.
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb09863.x
Subject(s) - neuropeptide y receptor , dorsal root ganglion , chemistry , agonist , peptide yy , receptor , intracellular , biophysics , endocrinology , medicine , calcium , neuropeptide , biology , biochemistry , dorsum , anatomy , organic chemistry
1 The identity of the neuropeptide Y (NPY) receptor associated with the observed inhibition of neuronal Ca 2+ currents ( I Ca ) in rat dorsal root ganglion (DRG) cells has been established on the basis of agonist responses to analogues and carboxy terminal (C‐terminal) fragments of the NPY molecule. 2 Whole cell barium currents ( I Ba ) in DRG cells were reversibly inhibited by 100 n m NPY, 100 n m PYY and C‐terminal fragments of NPY in a manner that correlated with the length of the NPY fragments (for inhibition of the I Ba NPY = PYY > NPY2–36 > NPY13–36 > NPY16–36 > NPY18–36 ≫ NPY25–36). 3 C‐terminal fragments of NPY were also effective in reversibly reducing the I Ca , the associated increase in the intracellular Ca 2+ concentration ([Ca 2+ ] i ) and the increased [Ca 2+ ] i produced by evoked action potentials in the DRG cells. In addition, a Ca 2+ ‐activated Cl − conductance was also reversibly reduced by NPY fragments only when accompanied by a reduction in Ca 2+ entry. 4 We conclude that the Y 2 receptor for neuropeptide Y is coupled to inhibition of Ca 2+ influx via voltage‐sensitive calcium channels in DRG cells.